FIG 4.
Vandetanib inhibits dengue virus in Huh7 cells and extends survival in infected mice. (A) Chemical structures and (B) ROCS overlay of spautin-1 and vandetanib. (C) Dose-response curves for spautin-1 and vandetanib for treatment of DENV2-infected Huh7 cells revealed IC50 values of 1.1 μM and 1.6 μM, respectively. Compound addition, viral growth, and cytotoxicity assays were performed as described in the legend to Fig. 2C. (D) Administration of 15 mg/kg vandetanib twice daily significantly extended survival of DENV2-infected AGB6 mice. Log rank (Mantel-Cox) test, P = 0.0044. N = 12 (vehicle treated) or N = 11 (vandetanib treated).