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. 2020 Nov 10;11(6):e02825-20. doi: 10.1128/mBio.02825-20

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Copyright © 2020 Chen et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 2 — Production of nisin in plasmid-based and chromosomally integrated expression systems. (A) Extracellular and intracellular precursor nisin (NisA) detected by Western blotting using anti-leader peptide antibody. (B) Antimicrobial activity assay. The supernatant of the culture was incubated with the purified protease NisP. The indicator strain is Micrococcus flavus. (C) MALDI-TOF MS data. The predicted mass of fully modified precursor nisin is 5,688 Da. (a) Wild-type strain NZ9000, which does not contain nisin biosynthetic genes; (b) NZ9000/pTLR3-nisABTC; (c) NZ9000 pseudo10::nisABTC; pseudo 10, an integration locus in the chromosome of L. lactis NZ9000.