FIG 6.

Distribution of HIV-1 RNAs relative to the centrosome. The expression of Gag-mCherry-RRE and Gag-YFP-CTE proviruses were induced by the addition of doxycycline, and cells were fixed at 8 h, 16 h, and 24 h postinduction. HIV-1 RNA was detected by RNAscope; Gag-YFP-CTE and Gag-mCherry-RRE RNA signals are shown in green and red, respectively. Centrosomes were detected by immunofluorescence with antibody to γ-tubulin. (A) Representative images showing HIV-1 RNA and centrosome signals. (B) The spatial relationship between Gag-mCherry RRE and Gag-YFP-CTE RNAs to centrosomes in the cytoplasm of the cell. The distances of individual Gag-mCherry-RRE and Gag-YFP-CTE RNAs to the two centrosomes detected from a representative cell are shown.