TABLE 5.
rDNA and satellite repeats as detected by RepeatExplorer, BLASTn and FISH.
45 rDNA |
5S rDNA |
CL82 (369 bp) |
CL229 (46bp) |
CL217 (126 bp) |
CL201 (172bp)e |
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ID | RE (+/−) | BLAST (+/−) | No. of FISH loci | RE (+/−) | BLAST (+/−) | No. of FISH loci | RE (+/−) | BLAST (+/−) | No. of FISH loci | RE (+/−) | BLAST (+/−) | No. of FISH loci | RE (+/−) | BLAST (+/−) | No. of FISH loci | RE (+/−) | BLAST (+/−) | No. of FISH loci |
IntA | + | + | 4 | + | + | 2 | + | + | 1 | + | + | 2 | + | + | 2 | – | – | 0 |
IntC | + | + | 4 | – | + | 2 | – | (+)b | 0 | – | (+)c | 0 | + | + | 2 | – | – | 0 |
PreA | + | + | 6 | + | + | 2 | + | + | 7 | – | (+)c | 0 | – | + | 0 | + | + | 2 |
PreC | + | + | 6 | + | + | 2 | + | + | 7 | – | (+)c | 0 | + | + | 0 | + | + | 2 |
PalA | + | + | 7 | + | + | 3 | + | + | 2 | + | + | 1 | + | + | 2 | – | (+)c | 1 |
PalF | + | + | 7 | + | + | 3 | + | + | 6 | + | + | 1 | + | + | 3+6d | – | (+)c | 0 |
PicF | + | + | 7a | + | + | 3 | + | + | 9 | + | + | 1 | + | + | 4+8d | + | + | 2 |
PicB | + | + | 7a | + | + | 3 | + | + | 6 | + | + | 1 | + | + | 2 | + | + | 2 |
Hyb2 | + | + | 5 | + | + | 2 | + | + | 4 | + | + | 1 | + | + | 1 | + | + | 2 |
Hyb3 | + | + | 5 | + | + | 2 | + | + | 4 | + | + | 1 | + | + | 1 | + | + | 1 |
Hyb4 | + | + | 5 | + | + | 2 | + | + | 6 | + | + | 1 | + | + | 1 | + | + | 2 |
ID = Sample ID; RE/BLAST (+/−) = RepeatExplorer/BLAST (cluster detected/cluster not detected).aThe numbers of loci for 45S rDNA in PicF and PicB were reported in Chrtek et al. (2020).bCL82 was detected in a very low amount in IntC (see Supplementary Table 3) and a closer inspection showed that the corresponding reads displayed a rather low similarity to the sequence of satellite CL82 (see section “Discussion”).csatellite repeat detected only in trace amounts (see Supplementary Table 3).dbesides major loci found on all metaphase plates (PalF 3, PicF 4), additional loci (PalF 6, PicF 8) of CL217 occurred in these samples (see section “Results” and Figure 6).eCL201 was present as two hemizygous loci on non-homologous chromosomes in Pre.