. 2020 Nov 2;11:584646. doi: 10.3389/fimmu.2020.584646

Table 1.

Phenotypic features used in the Manual Gating (MG) strategy for the identification of lymphoid populations in blood according to the EuroFlow guidelines for analysis of blood samples stained with PIDOT.

Population	Gating strategy ⁽¹⁾
B-cells	FSC^lo SSC^loCD45^hiCD19⁺CD3^-CD45RA⁺
Pre-germinal center B-cells	CD27^-IgD⁺IgM⁺
Post-germinal center B-cells/plasmacells (MBC/PC)
Unswitched MBC/PC ⁽²⁾	IgD⁺IgM⁺CD27 ⁺
Switched MBC/PC ⁽²⁾	IgD^-IgM^-CD27^{- to +}
IgD⁺IgM^- post-GC	IgD⁺IgM^-CD27⁺
T-cells	FSC^lo SSC^loCD45^hiCD3⁺CD19^-CD16&CD56^{- to lo}
TCRγδ⁺ T-cells	TCRγδ ⁺CD4^-CD8^{- to lo}
TCRγδ^- CD4^-CD8^- T-cells	TCRγδ ^-CD4^-CD8^{- to lo}
CD4⁺ T-cells	TCRγδ ^-CD4⁺CD8^-
CD4⁺ naive T-cells	CD27⁺CD45RA⁺
CD4⁺ central memory T-cells	CD27⁺CD45RA^-
CD4⁺ effector memory T-cells	CD27^-CD45RA^-
CD4⁺ terminal effector T-cells	CD27^-CD45RA⁺
CD8⁺ T-cells	TCRγδ ^-CD4^-CD8⁺
CD8⁺ naive T-cells	CD27⁺CD45RA⁺
CD8⁺ central memory T-cells	CD27⁺CD45RA^-
CD8⁺ effector memory T-cells	CD27^-CD45RA^-
CD8⁺ effector CD27+ T-cells	CD27^loCD45RA⁺
CD8⁺ terminal effector T-cells	CD27^-CD45RA⁺
CD4⁺CD8⁺ T-cells	TCRγδ ^-CD4⁺CD8⁺
Natural Killer cells	SSC-A^loFSC-A^loCD45^hiCD19^-CD3^-CD16&CD56^hiCD45RA^{lo to +}

⁽¹⁾In addition to the classical two-dimensional gating based on the listed markers, automatic population separator (APS) plots were used for fine-tune the gating of the listed cell populations as described elsewhere (4); ⁽²⁾Most MBC/PC, but not all, are CD27⁺.