Table 3.
Correlation between absolute counts obtained by manual gating (MG) versus automated gating and identification (AG&I).
| Healthy donors | Patients | Total | |
|---|---|---|---|
| Lymphocytes | 0.996 | 0.994 | 0.994 |
| B-cells | 0.996 | 0.998 | 0.998 |
| Pre-GC B-cells | 0.997 | 0.992 | 0.995 |
| Unswitched MBC/PC | 0.955 | 0.845 | 0.877 |
| Switched MBC/PC | 0.983 | 0.963 | 0.956 |
| T-cells | 0.997 | 0.992 | 0.992 |
| CD4+ T-cells | 0.998 | 0.989 | 0.990 |
| CD4+ naive T-cells | 0.976 | 0.988 | 0.989 |
| CD4+ central memory T-cells | 0.960 | 0.933 | 0.923 |
| CD8+ T-cells | 0.995 | 0.980 | 0.991 |
| CD8+ naive T-cells | 0.987 | 0.978 | 0.983 |
| CD8+ central memory T-cells | 0.779 | 0.933 | 0.893 |
| TCRγδ+ T-cells | 0.999 | 0.925 | 0.942 |
| TCRγδ- CD4- CD8- T-cells | 0.951 | 0.915 | 0.955 |
| Natural killer cells | 0.883 | 0.983 | 0.973 |
Results expressed as Spearman rank correlation coefficient values for those cell populations that are mandatory for PID screening and classification according to the ESID criteria. For all correlations p-vales < 0.001 were detected.