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. 2020 Nov 2;10:587708. doi: 10.3389/fcimb.2020.587708

Table 1.

Primers used to amplify the specific genes in this study.

Gene target Primer name Forward primer (5′→3′) Reverse primer (5′→3′) Expected length (bp)
Vertebrate mtDNA cytb BM1/BM2 CCCCTCAGAATGATATTTGTCCTCA (Boakye et al., 1999) CCATCCAACATCTCAGCATGATGAAA (Boakye et al., 1999) 358
Trypanosoma cruzi nDNA satellite repeat TCZ1/TCZ2 CGAGCTCTTGCCCACACGGGTGCT (Moser et al., 1989) CCTCCAAGCAGCGGATAGTTCAGG (Moser et al., 1989) 188
Trypanosoma cruzi minicircle kDNA Tc121/Tc122 AAATAATGTACGGG(T/G)GAGATGCATGA (Wincker et al., 1994) GGTTCGATTGGGGTTGGTGTAATATA (Wincker et al., 1994) 330