FIGURE 1.

Nanobodies improve penetration and detect endogenous proteins. (A) Anti-GFP nanobody labeling (mCherry and peroxidase fused) and IgG labeling in H2B-GFP expressing cells. Cells permeabilized for 5 min with 0.1% Triton before labeling. Nanobodies and primary and secondary antibodies incubated for 1 h each. Note the colozalization between GFP and mCherry (nanobody), most prominently in the low-expressing cells, while Alexa Fluor 594 (IgG) mainly localizes in the cytoplasm. (B) High HER2 expressing cells, SkBr3, labeled with nanobodies targeting HER2. Overlay of nanobody fluoresence and EM image. Note the positive labeling at cell-cell contact sites. Bars: 10 μm. Reproduced from De Beer et al. (2018), http://creativecommons.org/licenses/by/4.0/.