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. 2020 Nov 2;14:573278. doi: 10.3389/fncel.2020.573278

TABLE 2.

Nanobody implemented in microscopy – An overview of targets that have been visualized using nanobodies and microscopy.

Target Delivered as Technique References
Actin cDNA or Protein LM / SR Rocchetti et al., 2014; Panza et al., 2015; Plessner et al., 2015; Moutel et al., 2016; Periz et al., 2017; Wegner et al., 2017; Abdellatif et al., 2019; Tosetti et al., 2019
Active β2-Ars cDNA LM Irannejad et al., 2013
Alexandrium Minutum cDNA LM Mazzega et al., 2019
ALFA-tag cDNA or Protein LM / SR Gotzke et al., 2019
AMIGO-1 cDNA LM Dong et al., 2019
Amyloid β Protein LM Li et al., 2016
Arabidopsis Thaliana Protein EM De Meyer et al., 2014
ARTC2 Protein LM Bannas et al., 2015a,b
ATP7B cDNA LM Huang et al., 2014
bacteriophage p2 Protein EM De Haard et al., 2005
BC2-tag Protein LM / SR Braun et al., 2016; Virant et al., 2018
β-catenin cDNA or Protein LM Traenkle et al., 2015; Hebbrecht et al., 2020
BFP Protein SR Sograte-Idrissi et al., 2019
CapG cDNA LM De Clercq et al., 2013; Van Audenhove et al., 2013; Van Impe et al., 2008
CD11b Protein LM / CLEM Rashidian et al., 2015; Duarte et al., 2016; Fang et al., 2018; Wöll et al., 2018; Cheloha et al., 2019
CEA Protein LM Hafian et al., 2014; Ramos-Gomes et al., 2018
C.Jejuni Protein LM Riazi et al., 2013
Clostridium Difficile toxin Protein LM Pizzo et al., 2018
Cortactin cDNA LM Van Audenhove et al., 2014, 2015; Bertier et al., 2018; Hebbrecht et al., 2020
Ebolavirus Protein LM Darling et al., 2017; Sherwood and Hayhurst, 2019
EGFR Protein LM / CLEM Iqbal et al., 2010a; Oliveira et al., 2012; Van De Water et al., 2012; Zarschler et al., 2014; Kooijmans et al., 2016; Krüwel et al., 2016; Van Driel et al., 2016; De Beer et al., 2018; Beltrán Hernández et al., 2019; Karges et al., 2019
Eps15 cDNA LM Traub, 2019
Extracellular vesicles Protein EM Popovic et al., 2018
Fascin cDNA LM Van Audenhove et al., 2015; Gross et al., 2016
FGFR1 cDNA LM Monegal et al., 2009
γ-H2Ax cDNA LM Rajan et al., 2015
GPCR cDNA SR Sungkaworn et al., 2017
Gelsolin cDNA LM Van Impe et al., 2008; Van Den Abbeele et al., 2010; De Clercq et al., 2013; Van Audenhove et al., 2013
Gephyrin cDNA LM Dong et al., 2019
GFAP Protein LM / CLEM Li et al., 2012; Fang et al., 2018
GFP / YFP cDNA or Protein LM / SR/CLEM Rothbauer et al., 2006, 2008; Bazl et al., 2007; Schornack et al., 2009; Kirchhofer et al., 2010; Caussinus et al., 2011; Casas-Delucchi et al., 2012; Han et al., 2012; Li et al., 2012; Pellis et al., 2012; Ries et al., 2012; Herce et al., 2013, 2017; Szymborska et al., 2013; Truan et al., 2013; Bleck et al., 2014; Wang et al., 2014; Ariotti et al., 2015, 2017, 2018; Finnigan et al., 2015; Harmansa et al., 2015, 2017; Kamiyama et al., 2015; Kaplan and Ewers, 2015; Katoh et al., 2015; Klamecka et al., 2015; Nieuwenhuizen et al., 2015; Platonova et al., 2015a,b; Roebroek et al., 2015; Shin et al., 2015; Wedeking et al., 2015; Berry et al., 2016; Chamma et al., 2016, 2017; Drees et al., 2016; Gross et al., 2016; Harper et al., 2016; Joensuu et al., 2016; Künzl et al., 2016; Moutel et al., 2016; Tang et al., 2016a,b; Teng et al., 2016; Wendel et al., 2016; Katrukha et al., 2017; Roder et al., 2017; Almuedo-Castillo et al., 2018; Buser et al., 2018; De Beer et al., 2018; Gadok et al., 2018; Klein et al., 2018; Pani and Goldstein, 2018; Temme et al., 2018; Buser and Spiess, 2019; Cramer et al., 2019; Ghosh et al., 2019; Mann et al., 2019; Osswald et al., 2019; Prole and Taylor, 2019; Seitz and Rizzoli, 2019; Sograte-Idrissi et al., 2019; Farrants et al., 2020; Joshi et al., 2020
Gp41 (HIV) cDNA LM Lutje Hulsik et al., 2013; Boersma et al., 2019
H2A-H2B cDNA LM Jullien et al., 2016
HER2 Protein LM / CLEM Kijanka et al., 2013; Rakovich et al., 2014; Zou et al., 2015; D’Hollander et al., 2017; Kijanka et al., 2017; De Beer et al., 2018; Ramos-Gomes et al., 2018; Martinez-Jothar et al., 2019; Debie et al., 2020
HIF-1α Protein LM Groot et al., 2006
HIV-1 cDNA LM / SR Helma et al., 2012
Heterochromatin Protein 1α cDNA or Protein LM Moutel et al., 2016
Homer1 cDNA or Protein LM / SR Dong et al., 2019
Human Neonatal Fc Receptor Protein LM Andersen et al., 2013
Huntingtin cDNA LM Southwell et al., 2008; Maiuri et al., 2017
IGFBP7 Protein LM Iqbal et al., 2010b; Tomanek et al., 2012
IRSp53 cDNA LM Dong et al., 2019
Lamin cDNA or Protein LM / SR Rothbauer et al., 2006; Schmidthals et al., 2010; Roder et al., 2017; Klein et al., 2018
L-plastin cDNA LM Delanote et al., 2010; De Clercq et al., 2013
LY-6C/6G Protein CLEM Fang et al., 2018
Marburgvirus Protein LM Darling et al., 2017; Sherwood and Hayhurst, 2019
MHC II Protein LM Rashidian et al., 2015; Duarte et al., 2016; Cheloha et al., 2019
Mouse IgG Protein LM / SR Pleiner et al., 2018
NFT2 cDNA LM Van Audenhove et al., 2013
NTA domain cortactin cDNA LM Bertier et al., 2018
Nup35 Protein SR Ma et al., 2017
Nup37 Protein SR Ma et al., 2017
Nup85 Protein LM / SR Pleiner et al., 2015
Nup93 Protein LM / SR Pleiner et al., 2015; Göttfert et al., 2017
Nup98 Protein LM / SR Pleiner et al., 2015; Göttfert et al., 2017
Nup155 Protein LM / SR Pleiner et al., 2015
n-WASP cDNA LM Hebbrecht et al., 2017
p53 cDNA or Protein LM Moutel et al., 2016
PARP1 cDNA LM Buchfellner et al., 2016
PCNA cDNA LM Burgess et al., 2012; Panza et al., 2015; Schorpp et al., 2016
PepTag cDNA LM Traenkle et al., 2020
PFR1 Protein LM Obishakin et al., 2014
POM121 Protein SR Ma et al., 2017
PSMA Protein LM Fan et al., 2015
Rabbit IgG Protein LM / SR Pleiner et al., 2018
RFP / mCherry cDNA or Protein LM / SR / CLEM Ries et al., 2012; Bleck et al., 2014; Platonova et al., 2015a,b; Wedeking et al., 2015; Moutel et al., 2016; Teng et al., 2016; Ariotti et al., 2018; Buser et al., 2018; Buser and Spiess, 2019; Cramer et al., 2019; Prole and Taylor, 2019; Sograte-Idrissi et al., 2019
SAPAP2 cDNA LM Dong et al., 2019
NAP-25 Protein LM / SR Maidorn et al., 2019
Survivin cDNA LM Beghein and Gettemans, 2017
Syntaxin 1A Protein LM / SR Maidorn et al., 2019
Tau (phosphorylated) Protein LM Li et al., 2016
Tubulin cDNA or Protein LM / SR Olichon and Surrey, 2007; Mikhaylova et al., 2015; Moutel et al., 2016
VEGFR cDNA LM Ahani et al., 2016
Vimentin cDNA or Protein LM / SR Maier et al., 2015, 2016; Klein et al., 2018
VSG Protein LM Stijlemans et al., 2004
Vsig4 Protein LM Zheng et al., 2019
Vγ9Vδ2-T cell Protein LM De Bruin et al., 2016

EM, electron microscopy; SR, super resolution fluorescence microscopy; CLEM, correlated LM/EM.