FIGURE 3.

ACP protected dopaminergic neurons by inhibiting 6‐OHDA‐induced ROS expression. (a, d) ACP exerted a protective effect on 6‐OHDA‐induced dopaminergic neurons. ACP intervention led to significantly down‐regulated apoptotic rate, compared with Con group, *p < .05; compared with 6‐OHDA group, #p < .05. (b, c) DCFH‐DA staining was used to detect ROS expression. The cellular level of ROS was significantly down‐regulated after ACP intervention, and the number of positive cells decreased, as indicated by flow cytometry. (e) The results of fluorescence intensity after DCFH‐DA staining: The absorbance was significantly down‐regulated after ACP intervention in a dose‐dependent manner, compared with Con group, *p < .05; compared with 6‐OHDA group, #p < .05