FIGURE 4.

Effect of protein and mRNA expression of NPRP3 after ACP intervention. (a, b) ACP pretreatment led to significantly down‐regulated 6‐OHDA‐induced NLRP3 activation. In addition, the expression of NLRP3, ASC, and caspase‐1 was down‐regulated, while that of pro‐caspase‐1 was up‐regulated, and the level of pro‐caspase‐1 was decreased, *p < .05 compared to Con group; #p < .05 compared to 6‐OHDA group. (b, c) ACP pretreatment resulted in the significantly down‐regulated levels of inflammatory factors IL‐1β and IL‐18 in the culture medium, compared with Con group, *p < .05; compared with 6‐OHDA group, #p < .05. (d) ACP pretreatment caused down‐regulation in the mRNA expression levels of NLRP3, ASC, caspase‐1, and IL‐1β, which was consistent with protein level trend, compared with Con group, *p < .05; compared with 6‐OHDA group, #p < .05