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. 2020 Nov 2;10:1688. doi: 10.3389/fonc.2020.01688

FIGURE 2.

FIGURE 2

Activation of LXR-α inhibits prostate cancer progression and metastasis. (A) Effects of LXRs’ overexpression on the expression of cell progression-related protein in PC3 cell line. (B) Overexpression of LXR-α significantly inhibited cell proliferation in the PC3 cell line. Cell viability was detected with Cell Counting Kit-8 (CCK-8) assay. (C) Overexpression of LXR-α significantly suppressed colony formation in the PC3 cell line. (D) Overexpression of LXR-α extremely decreased cell invasion ability of the PC3 cell line. (E) Compared with LXR-α transfected alone, co-treatment with GW3965 could better inhibit cell proliferation. Cell viability was detected with CCK-8 assay. (F) Overexpression of LXR-α with concomitant treatment with GW3965 resulted in a greater inhibition of the clone-forming ability of PC3 cell line than GW3965 alone. (G) LXR-α overexpression decreased cell invasion ability of PC3 cell line, and did so more significantly through co-treatment with GW3965. (H) Compared with LXR-α transfected alone, co-treatment with GW3965 could better increase p21, p27, E-cadherin, and C-Caspase 3. Data were presented as means ± SD. ∗∗p < 0.01, ∗∗∗p < 0.001, compared with the control group; n = 3. GAPDH levels served as the control for equal loading.