Table 4.
Unfolding temperatures of CK2α in the presence of compound 6, 9 or CX-4945, as monitored by thermal denaturation of protein by circular dichroism (TdCD).
| Entry | Sample | Tm (°C) |
|---|---|---|
| 1 | CK2α | 53.2 (± 0.3) |
| 2 | CK2α + 6 (50 μM) | 50.3 (± 0.3) |
| 3 | CK2α + 9 (6.25 μMa) | 52.0 (± 0.2) |
| 4 | CK2α + CX-4945 (6.25 μMa) | 55.4 (± 0.2) |
The midpoint unfolding temperatures (Tm) are averages of three independent experiments (± SD). CK2α concentration was 5 μM.
a
Concentrations were limited by the compound solubility in methanol.