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. Author manuscript; available in PMC: 2020 Nov 16.
Published in final edited form as: Biochem Biophys Res Commun. 2014 Feb 1;444(4):644–650. doi: 10.1016/j.bbrc.2014.01.129

Fig. 3.

Fig. 3.

Determination of centriole lengths in CPAP-depleted mitotic cells. (A) Mitotic HeLa cells were coimmunostained with antibodies specific to CEP135 (red), CP110 (green) and SAS-6 (green), and the spindle poles were observed with a super-resolution microscope. Distance between red (CEP135) and green (CP110) dots were determined. Procentrioles were identified with two consecutive green dots. Nineteen centrioles were analyzed and the data were presented as means and standard errors. (B) The distance between CEP135 (red) and CP110 (green) signals was determined in CPAP-depleted mitotic cells. Over 60 centrioles per group were analyzed in three independent experiments *P < 0.05. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)