Fig. 1.

(A) Microscopic observation (×400) of human malignant pleural mesothelioma (HMPM) cell lines (211H, H2452 and H226), a mesothelial cell line (Met5A) 72 h after infection with fusion gene deleted non-transmissible Sendai virus vector encoding FUSE-binding protein-interacting repressor (SeV/ΔF/FIR) or fusion gene deleted non-transmissible Sendai virus vector encoding green fluorescent protein (SeV/ΔF/GFP) at 100 multiplicity of infection (MOI), and the control (PBS). GFP-transduced cells were observed under fluorescence microscopy. SeV/ΔF/FIR induced significant reduction in cells. (B) Transduction efficiency of 211H, H2452, H226 and Met5A with SeV/ΔF/GFP at 10, 100 and 1000 MOI. The bar depicts the percentage of GFP-positive cells at 72 h after transduction with SeV/ΔF/GFP (error bars, SD). (C) Endogenous expression of FIR, c-Myc and P89 among three HMPM cells and immortalized non-tumor mesothelial cells. β-actin was used as the loading control. (D) Cytotoxic activities of SeV/ΔF/FIR in 211H, H2452, H226 and Met5A. Cells were infected with different doses of SeV/ΔF/FIR (■) or SeV/ΔF/GFP (♦) at 10, 100 and 1000 MOI; a MTS cell viability assay was performed 72 h after infection. Relative cell viability was calculated based on the value of uninfected cells as 100% by the MTS assay (error bars, SD). One hundred MOI of SeV/ΔF/FIR for 72 h was sufficient to induce apoptosis.