QPCR analyses of FSP27 (A) and PPARγ (B) mRNAs were performed in each genotyped mouse liver. Expression was normalized to 36B4 mRNA, and each bar represents the average±S.E.M. of three separate experiments. Normal, normal genetic background mice; ob/ob, leptin-deficient mice; WT, PPARγ wild-type mice liver; KO, PPARγKO mice liver. Note: ob/ob-PPARγWT mice have fatty liver, whereas ob/ob-PPARγKO mice are normal or have much less fat.7) Significant differences from PPARγWT liver: * p<0.01, ** p<0.001.