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. Author manuscript; available in PMC: 2021 May 1.
Published in final edited form as: Methods. 2020 Feb 5;177:35–49. doi: 10.1016/j.ymeth.2020.02.002

Figure 3. Flowchart of EV separation from unfixed murine brain tissue via SG-UC and SEC methods.

Figure 3.

Sucrose gradient ultracentrifugation (SG-UC) or Size exclusion chromatography (SEC) protocols for separating of EVs from unfixed mouse frozen brain tissue. See section 3.1 for detailed methods. TEM image of frozen mouse brain-derived EVs (pooled fractions V and VI for SG-UC and pooled fraction 8 and 9 for SEC). Scale bar; 100 nm.