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. Author manuscript; available in PMC: 2021 May 1.
Published in final edited form as: Methods. 2020 Feb 5;177:35–49. doi: 10.1016/j.ymeth.2020.02.002

Table 1.

Assessment of tissue homogenates, pellet at 10,000g, sucrose fraction III, IV, V, VI and VII separated from human brain tissue by SG-UC method

Tissue homogenates Pellet at 10,000g Fraction III Fraction IV Fraction V Fraction VI Fraction VII
Mode size (nm)a 113 139 172 148 138 139 119
Particle numbera,b 1.31E+12 3.21E+11 5.09E+10 1.42E+11 1.52E+11 6.77E+10 1.12E+10
Total protein (μg)b,c 3672 352.1 25.6 56.1 80.3 85.1 24.3
Particles/proteins (μg) 3.57E+08 9.12E+08 1.99E+09 2.53E+09 1.89E+09 7.96E+08 4.61E+08
a

Particle number and size of separated EVs were measured by Nanoparticle Tracking Analysis (NTA)

b

The starting material is 0.5g of human brain cortex grey matter

c

The total protein levels were measured by BCA assay.