Table 2.
Assessment of EVs separated from unfixed frozen murine brain tissue by SG-UC and SEC methods
| SG-UC Fraction V | SG-UC Fraction Vl | SEC Fraction 8 | SEC Fraction 9 | |
|---|---|---|---|---|
| Mode size (nm)a | 135 | 139 | 90 | 127 |
| Particle numbera,b | 1.06E+10 / 30μL | 1.09E+10 / 30μL | 6.80E+10 / 500μL | 7.80E+10 / 500μL |
| Total protein (μg)b,c | 43.3 | 41.1 | 44.1 | 59.3 |
| Particles/proteins (μg) | 2.44E+08 | 2.65E+08 | 1.54E+09 | 1.31E+09 |
| Protein identificationd | 933 | 1823 | ||
| Extracellular Exosomee | 489 (52.4%) | 827 (45.4%) | ||
a
Particle number and size of separated EVs were measured by Nanoparticle Tracking Analysis (NTA)
b
The starting material is 0.5g of human brain cortex grey matter
c
The total protein levels were measured by BCA assay.