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. Author manuscript; available in PMC: 2021 May 1.

Published in final edited form as: Methods. 2020 Feb 5;177:35–49. doi: 10.1016/j.ymeth.2020.02.002

Table 3.

Assessment of EVs separated from human CSF by AC method

	Human CSF	EV Fraction
Mode size (nm)^a	162	114
Particle number^a,b	7.9E+9 / 500μL CSF	2.15E+9 / 100μL
Total protein (μg)^b,c	1125.73	1.78
Protein identification^d	-	700
Particles/proteins (μg)	7.02E+06	1.21E+09

^a

Particle number and size of separated EVs were measured by Nanoparticle Tracking Analysis (NTA)

^b

The starting material is 500ml of human CSF

^c

The total protein levels were measured by BCA assay

^d

The proteins were identified by LC-MS/MS for EV fraction, but CSF was not analyzed by LC-MS/MS.