Figure 1.

Characterization of rat bone marrow cells. Differentiation of RBM cells in induction media for osteogenic and adipogenic differentiation. (A) Established RBM cells after 2 passages were used as control for confirmation of differentiation. (B) Differentiation to adipogenic lineage was confirmed with lipid condensations through cell cytoplasm stained by Oil red, 14 days after culture in induction medium compared to RBM cells. (C) Calcified structures were evaluated in osteocyte stained by Alizarin red, 21 days after culture in induction medium. (D) Evaluation of RBM cell markers expression showed more than 95% in CD90, CD105 and less than 0.5% in CD45 and CD34 markers by flowcytometry which verified stem cell property of RBM cells