Figure 7.

The effect of intranasal EV-loaded NEP administration on beta-amyloid plaques accumulation. (A) Representative micrographs of immunofluorescence staining of beta-amyloid proteins in Normal, Control, EVs, and EV-loaded NEP groups, Scale bar: 50μm. Cell nuclei were counterstained with DAPI and (B) Expression percentage of Aβ plaques, significantly decreased in EV-loaded NEP group was observed in comparison to control (p < 0.001) and EVs (p < 0.01) groups after 14 days treatment. Images were represented from at least 3 sections per animal in each experimental group after 14 days treatment; (n = 4 in each group, the results were quantified with ImageJ software and the differences between groups were determined by ANOVA followed by Tukey test). Defined groups are Normal, Ctrl: received PBS, EVs: received EVs, EV&NEP: received EV-loaded NEP in the treatment period