Table 3. Methods used by national reference or expert laboratories for species identification, phenotypic carbapenem susceptibility testing and genotypic characterisation of carbapenem-resistant Acinetobacter baumannii (CRAb), European countries, 2019 (nā=ā37).
Country | Method for species determination |
Method for phenotypic carbapenem susceptibility testing |
Method for genotypic characterisation of carbapenem resistance |
|||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Biochemical | MALDI-TOF | PCR/gene sequencing | WGS | Automated system | Disc diffusion | Gradient test | Commercial broth microdilution | Agar dilution | In-house broth microdilution | PCR | Real-time PCR | Single-gene sequencing | WGS | Other | ||
Albania | Yes | No | No | No | Yes | Yes | Yes | No | No | No | Not performed | |||||
Austria | Yes | Yes | Yes | No | No | Yes | Yes | Yes | No | No | Yes | No | No | No | Yesa | |
Belgium | No | Yes | Yes | Yes | No | Yes | No | Yes | No | No | Yes | No | Yes | Yes | No | |
Bosnia and Herzegovinab | No NRL or expert laboratory | |||||||||||||||
Bulgaria | Yes | No | Yes | No | No | Yes | No | Yes | No | No | Yes | No | No | No | No | |
Croatia | No NRL or expert laboratory | |||||||||||||||
Cyprus | No NRL or expert laboratory | |||||||||||||||
Czechia | Yes | Yes | No | No | No | Yes | Yes | Yes | No | No | Yes | No | Yes | Yes | No | |
Denmark | No | Yes | Yes | Yes | No | Yes | Yes | Yes | Yes | No | No | No | No | Yes | No | |
Estonia | No NRL or expert laboratory | |||||||||||||||
Finland | No | No | No | Yes | Yes | Yes | Yes | No | No | No | No | No | No | Yes | No | |
France | No | Yes | Yes | No | No | Yes | Yes | Yes | No | Yes | Yes | No | Yes | Yes | No | |
Germany | No | Yes | Yes | No | No | Yes | Yes | Yes | No | No | Yes | No | Yes | Yes | No | |
Greece | No NRL or expert laboratory | |||||||||||||||
Hungary | No | Yes | Yes | No | No | Yes | Yes | No | No | Yes | Yes | No | No | Yes | No | |
Iceland | No | Yes | Yes | No | No | Yes | Yes | Yes | No | No | Yes | Yes | No | Yes | Yesc | |
Ireland | No | Yes | No | No | No | No | Yes | No | No | No | No | Yes | No | No | No | |
Italyd | Yes | No | Yes | Yes | No | Yes | Yes | Yes | No | No | Yes | No | Yes | Yes | No | |
Kosovoe | Yes | No | No | No | Yes | Yes | Yes | Yes | No | No | Not performed | |||||
Latvia | Yes | Yes | No | No | Yes | No | Yes | No | No | No | No | Yes | No | No | No | |
Lithuania | No | Yes | No | No | No | Yes | No | Yes | No | No | Not performed | |||||
Luxembourg | No | Yes | No | No | No | Yes | No | Yes | No | No | No | Yes | No | Yes | No | |
Malta | No | Yes | No | No | Yes | No | No | No | No | No | Not performed | |||||
Montenegro | No NRL or expert laboratory | |||||||||||||||
The Netherlands | No | Yes | No | No | Yes | No | No | No | No | No | Yes | No | No | Yes | No | |
North Macedonia | Yes | N | No | No | Yes | Yes | Yes | No | No | No | No | Yes | No | No | No | |
Norway | No | Yes | Yes | Yes | No | No | No | Yes | No | No | Yes | No | No | Yes | No | |
Poland | Yes | No | No | No | No | Yes | Yes | No | No | Yes | Yes | No | No | No | No | |
Portugal | No | Yes | Yes | No | No | Yes | No | No | No | Yes | Yes | No | Yes | Yes | No | |
Romania | Yes | Yes | Yes | No | No | Yes | No | No | No | No | Yes | No | Yes | No | No | |
Serbia | No | Yes | No | No | No | Yes | Yes | Yes | No | No | Yes | No | No | No | No | |
Slovakia | No NRL or expert laboratory | |||||||||||||||
Slovenia | No | Yes | Yes | No | No | Yes | Yes | Yes | No | No | Yes | Yes | No | Yes | No | |
Spain | Yes | Yes | Yes | Yes | No | Yes | Yes | Yes | No | Yes | Yes | Yes | Yes | Yes | No | |
Sweden | No | Yes | No | Yes | No | No | No | Yes | No | No | No | No | No | Yes | No | |
Turkey | No | Yes | Yes | No | No | Yes | Yes | No | Yes | Yes | Yes | No | No | No | No | |
UK | England | No | Yes | Yes | No | No | No | Yes | Yes | No | No | Yes | Yes | No | No | No |
Northern Ireland | See UK ā England | |||||||||||||||
Scotland | Yes | No | No | No | No | No | No | Yes | No | No | Not performed | |||||
Wales | No | Yes | No | Yes | No | Yes | Yes | No | No | Yes | Yes | Yes | No | Yes | No |
MALDI-TOF: matrix-assisted laser desorption/ionization-time of flight mass spectrometry; NRL: national reference laboratory; PCR: polymerase chain reaction; UK: United Kingdom; WGS: whole genome sequencing.
a Commercial multiplex nucleic acid amplification methods.
b Results reported for Bosnia and Herzegovina only apply to Republic of Srpska.
c Commercial loop-mediated isothermal amplification.
d Species determination, phenotypic and genotypic characterisation are not routinely performed but only upon request.
e This designation is without prejudice to positions on status, and is in line with United Nations Security Council resolution 1244/99 and the International Court of Justice Opinion on the Kosovo declaration of independence.