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. 2020 Nov 12;25(45):2001735. doi: 10.2807/1560-7917.ES.2020.25.45.2001735

Table 3. Methods used by national reference or expert laboratories for species identification, phenotypic carbapenem susceptibility testing and genotypic characterisation of carbapenem-resistant Acinetobacter baumannii (CRAb), European countries, 2019 (nā€‰=ā€‰37).

Country Method for
species determination
Method for phenotypic
carbapenem susceptibility testing
Method for genotypic characterisation
of carbapenem resistance
Biochemical MALDI-TOF PCR/gene sequencing WGS Automated system Disc diffusion Gradient test Commercial broth microdilution Agar dilution In-house broth microdilution PCR Real-time PCR Single-gene sequencing WGS Other
Albania Yes No No No Yes Yes Yes No No No Not performed
Austria Yes Yes Yes No No Yes Yes Yes No No Yes No No No Yesa
Belgium No Yes Yes Yes No Yes No Yes No No Yes No Yes Yes No
Bosnia and Herzegovinab No NRL or expert laboratory
Bulgaria Yes No Yes No No Yes No Yes No No Yes No No No No
Croatia No NRL or expert laboratory
Cyprus No NRL or expert laboratory
Czechia Yes Yes No No No Yes Yes Yes No No Yes No Yes Yes No
Denmark No Yes Yes Yes No Yes Yes Yes Yes No No No No Yes No
Estonia No NRL or expert laboratory
Finland No No No Yes Yes Yes Yes No No No No No No Yes No
France No Yes Yes No No Yes Yes Yes No Yes Yes No Yes Yes No
Germany No Yes Yes No No Yes Yes Yes No No Yes No Yes Yes No
Greece No NRL or expert laboratory
Hungary No Yes Yes No No Yes Yes No No Yes Yes No No Yes No
Iceland No Yes Yes No No Yes Yes Yes No No Yes Yes No Yes Yesc
Ireland No Yes No No No No Yes No No No No Yes No No No
Italyd Yes No Yes Yes No Yes Yes Yes No No Yes No Yes Yes No
Kosovoe Yes No No No Yes Yes Yes Yes No No Not performed
Latvia Yes Yes No No Yes No Yes No No No No Yes No No No
Lithuania No Yes No No No Yes No Yes No No Not performed
Luxembourg No Yes No No No Yes No Yes No No No Yes No Yes No
Malta No Yes No No Yes No No No No No Not performed
Montenegro No NRL or expert laboratory
The Netherlands No Yes No No Yes No No No No No Yes No No Yes No
North Macedonia Yes N No No Yes Yes Yes No No No No Yes No No No
Norway No Yes Yes Yes No No No Yes No No Yes No No Yes No
Poland Yes No No No No Yes Yes No No Yes Yes No No No No
Portugal No Yes Yes No No Yes No No No Yes Yes No Yes Yes No
Romania Yes Yes Yes No No Yes No No No No Yes No Yes No No
Serbia No Yes No No No Yes Yes Yes No No Yes No No No No
Slovakia No NRL or expert laboratory
Slovenia No Yes Yes No No Yes Yes Yes No No Yes Yes No Yes No
Spain Yes Yes Yes Yes No Yes Yes Yes No Yes Yes Yes Yes Yes No
Sweden No Yes No Yes No No No Yes No No No No No Yes No
Turkey No Yes Yes No No Yes Yes No Yes Yes Yes No No No No
UK England No Yes Yes No No No Yes Yes No No Yes Yes No No No
Northern Ireland See UK ā€“ England
Scotland Yes No No No No No No Yes No No Not performed
Wales No Yes No Yes No Yes Yes No No Yes Yes Yes No Yes No

MALDI-TOF: matrix-assisted laser desorption/ionization-time of flight mass spectrometry; NRL: national reference laboratory; PCR: polymerase chain reaction; UK: United Kingdom; WGS: whole genome sequencing.

a Commercial multiplex nucleic acid amplification methods.

b Results reported for Bosnia and Herzegovina only apply to Republic of Srpska.

c Commercial loop-mediated isothermal amplification.

d Species determination, phenotypic and genotypic characterisation are not routinely performed but only upon request.

e This designation is without prejudice to positions on status, and is in line with United Nations Security Council resolution 1244/99 and the International Court of Justice Opinion on the Kosovo declaration of independence.