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. 2020 Oct 27;10(26):12158–12173. doi: 10.7150/thno.52000

Figure 4.

Figure 4

(A) THP fluorescence images of MDA-MB-231 cells incubated with P60-ICG-THP at pH 6.5 and 7.4 for different time points (the images were collected from the video provided in the supporting material). Scale bar = 20 μm. (B) The minimum time of fluorescence disappearance of P60-ICG-THP incubated with MDA-MB-231 at different pH values (n = 3). (C) THP fluorescence images of MDA-MB-231 incubated with P60-ICG-THP after treating with a drop of pH = 6.5 buffer in the same culture dish, scale bar = 20 μm. (D) The confocal microscope images of MDA-MB-231 incubated with P60-ICG-THP at different time points at different pH levels. Cells were stained with DAPI (blue), THP (red), and ICG (green) excited at 405 nm, 561 nm and 633 nm, respectively. Scale bar = 20 μm. (E) Endosome/lysosome labeling by Lyso Tracker, scale bar = 20 μm. (F) Schematic illustration of the cellular uptake of two drugs triggered by an acidic pH.