Skip to main content
. 2020 Oct 26;10(26):12127–12143. doi: 10.7150/thno.52450

Figure 2.

Figure 2

3D scaffolds promote a diversity of cell function. A) Immunofluorescence staining of adhesion marker vinculin (green) in HCT116 cells cultured on 3D collagen-coated and uncoated scaffolds (scale bar = 200 µm). B) Localization of vinculin in (A). The dimensions of each scaffold were 1 cm × 1 cm × 500 µm. C) Schematic representation illustrating physiological changes to cells grown in 2D and 3D environments. D) Cell morphology of HUVECs grown in 2D and 3D environments (red (phalloidin) = F-actin, blue (DAPI) = nuclei, scale bar = 100 µm). E) Immunofluorescence staining of MMP2 (green) in HELFs grown in 2D and 3D environments (scale bar = 200 µm). F) Immunofluorescence staining of stem cell marker CD133 (green) in HCT116 cells grown in 2D and 3D environments (Scale bar = 200 µm). G) Immunofluorescence staining of proliferation marker Ki67 (green) in HCT116 cells grown in 2D and 3D environments (scale bar = 200 µm). H-I) Gene expression of CD133 (H) and Ki6 (I) in HCT116 cells grown in 2D and 3D environments (*p < 0.05). All values are expressed as means ± SD, n = 5.