Skip to main content
. 2020 Oct 25;10(26):11950–11962. doi: 10.7150/thno.49717

Figure 1.

Figure 1

IL-8 derived from GCMSCs promotes the stemness of GC cells. (A) Levels of CD44, Sall4, Nanog, Oct4, and N-cadherin in SGC-7901 were examined by western blot. (B, C) Transwell migration (scale bar, 100 μm) and sphere formation (scale bar, 50 μm) assays were performed in SGC-7901 following GCMSC-CM treatment. (D, E) Quantification of cell migration and sphere numbers. (F) The ALDH activity of SGC-7901 was tested by ALDEFLUOR analyses. An IL-8 neutralizing antibody was added to GCMSC-CM and incubated at room temperature for 1 h earlier. The concentration of IL-8 neutralizing antibody was 5 μg/mL (R&D Systems). Data in D and E represents the mean ± SD of three repeated experiments (n=3). GCMSCs were isolated from three different GC patients. **, P<0.01, ***, P<0.001.