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. 2020 Oct 30;10(26):12204–12222. doi: 10.7150/thno.47683

Figure 3.

Figure 3

T-MSC treatment increased serum IGF-1 level. A. Serum cytokines of T-MSC-treated mice and PBS controls in acute colitis model were measured by the antibody array test. Normalized signal values were analyzed by student t-test, cytokines with p<0.05, and signal value>500 are listed. B. Fluorescent signals of IGF-1 detected by a microarray scanner in the antibody array test. C. Serum IGF-1 expression was verified by ELISA. a. experimental layout of the T-MSC treatment model. b. mice serum samples were collected on day 6, day 8, and day 10, and IGF-1 levels were detected by ELISA. D. Immunohistochemical staining of IGF-1 in the liver. a. IHC staining of liver sections. b. IHC scores of IGF-1. Scale bar = 100 µm. E. RT-qPCR for relative mRNA expression (normalized to GAPDH) of Igf-1 and Igfbps (Igfbp1, Igfbp3, Igfbp4) in the liver. F. T-MSCs and mouse primary hepatocytes were co-cultured at different ratios in cell-cell contact or Transwell assays, and supernatant IGF-1 was measured. Data are expressed as mean ± SD. *p < 0.05 and **p < 0.01. mIGF-1: mouse IGF-1.