Figure 6.

TFRC is a crucial target gene for the YTHDF1 promotion of iron metabolism. (A,B) CCK-8 (A) and colony formation (B) assays were performed after shCON and YTHDF1- knockdown Detroit 562 cells were transfected with the GV272 or GV272-TRFC-WT plasmid. (C) Masses of the xenograft tumors harvested from different groups. Tumor weight was measured 12 days after injection. (D) Intracellular iron levels (nmol) were measured in the GV272- and GV272-TRFC-WT- transfected shCON and YTHDF1-knockdown Detroit 562 and FaDu cells. (E,F) Flow cytometry and quantification of Phen Green- and LDH-positive shCON and YTHDF1-knockdown Detroit 562 cells transfected with the GV272 or GV272-TRFC-WT plasmid showing intracellular Fe²⁺ levels (E) and ROS levels (F).