a. Comparison of gene expression in B6 and DBA mice for each lung MP subset. Scatter plots are showing genes with transcripts per million (TPM) > 4. Pale blue dots show genes with fold change (FC) 2 or higher, dark blue dots show genes with FC 4 or higher. N=2 for each cell type in both strains.
b. Inter-cellular and inter-strain comparison of highly differentially expressed genes. Heat map shows unsupervised hierarchical clustering of genes with FC 4 or higher between the strains. Values are row z-score.
c. Examples of strain specific genes included in the heat map in Fig. 2b. Bar graphs represent mean log2 transcripts per million (TPM+1) ± SD, N=2. Asterisks indicate statistical significance, * p-adj < 0.05, ** p-adj < 0.01, *** p-adj < 0.001 calculated by DESeq2 and multiple-testing corrected with Benjamini-Hochberg.