Table 2. Inhibition of DfrBs (Non-His-tagged) with TMP and Inhibitors 1, 3, 6, and 7.

	Ki (mM)a	Ki (μM)
Inhibitor:	TMP	1	3	6	7
DfrB1	0.38 ± 0.09	130 ± 98	46 ± 5	18 ± 6	62 ± 6
DfrB2	0.81 ± 0.08	49 ± 3	52 ± 14	10 ± 2	29 ± 1
DfrB3	0.45 ± 0.10	12 ± 2	17 ± 2	2.4 ± 0.4	6.4 ± 2.1
DfrB4	0.50 ± 0.07	35 ± 4	51 ± 1	13 ± 1	20 ± 2
DfrB5	1.3 ± 0.3	49 ± 9	42 ± 1	7.4 ± 0.1	29 ± 3
DfrB7	0.66 ± 0.11	52 ± 32	37 ± 10	7.1 ± 2.2	19 ± 7

^aK_i values were calculated according to experimentally determined IC₅₀ (Table S2 and Table S3). Values are given in mM for TMP and in μM for all other inhibitors.