. 2020 Sep 28;11(11):2261–2267. doi: 10.1021/acsmedchemlett.0c00393

Table 3. Kinetic Constants for the Dihydrofolate Reductase Activity of DfrBs (Non-His-tagged).

Dfr	K_M^DHF (μM)	K_M^NADPH (μM)	k_cat^DHF (s^–1)	k_cat^DHF/k_M^DHF (μM^–1·s^–1)
EcDfr	1.2^a	0.94^a	12.0^b^,^c	10
DfrB1	4.4 ± 0.5	5.8 ± 0.8	0.32 ± 0.006	0.073
DfrB2	2.3 ± 0.2	3.3 ± 0.3	0.41 ± 0.01	0.18
DfrB3	3.0 ± 0.5	1.3 ± 0.3	0.20 ± 0.01	0.067
DfrB4	4.6 ± 0.7	3.5 ± 0.5	0.28 ± 0.01	0.061
DfrB5	3.2 ± 0.4	3.1 ± 0.2	0.32 ± 0.01	0.10
DfrB7	3.4 ± 0.4	2.8 ± 0.3	0.24 ± 0.007	0.071

Values taken from ref (41).

Value taken from ref (42).

k_cat value calculated for the global reaction.