Figure 5.

Cytotoxicity of phenothiazine analogues 1–5 toward cultured FRDA lymphocytes after incubation for 24 h in glucose-free (galactose) media to force the cells to rely on mitochondrial ATP production. Flow cytometric determination of cell viability by dual fluorescence labeling employed calcein-AM and ethidium homodimer-1 as live and dead stains, respectively. The actual percentage of live cells was extracted from a two-dimensional color density dot blot. Results obtained were verified by running duplicates in two independent experiments.