Table 1. Antiferroptotic Effects of MB/MV Analogues (1–5) in FRDA Lymphocytes against RSL3-Induced Lipid Peroxidation^a.

Compound	RSL3 challenge lipid peroxidation inhibition EC50 (nM)
MB	88 ± 13
Acetylated MBb	>2500
MV	26 ± 3
1a	76 ± 6
1b	14 ± 1
2a	53 ± 3
2b	12 ± 1
3a	38 ± 3
3b	9 ± 1
4a	54 ± 5
4b	16 ± 1
5a	70 ± 5
5b	25 ± 3
Lip-1	22 ± 2
Fer-1	48 ± 2
α-TOH	>500

^aThe calculated EC₅₀ values were determined graphically from the dose–response curves using the nonlinear regression curve fit analysis model.

^bN-acetyl leucomethylene blue (Figure 2).