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TM-P2-Thal and TM-P4-Thal degrade SIRT2 selectively in cells. (A) Immunoblots for SIRT1, SIRT2, and SIRT3 after treating with indicated concentrations of inhibitors for 48 h in MCF7 and BT-549 cells. (B) Immunoblot for SIRT2 after MCF7 cells were treated with indicated concentrations of TM-P4-Thal for 48 h. (C) Immunoblot for SIRT2 after MCF7 cells were treated with 10 μM of TM-P4-Thal for the indicated incubation times.
