. 2020 Sep 13;11(11):2318–2324. doi: 10.1021/acsmedchemlett.0c00444

Table 2. CXCR4 Antibody Binding Assay and Calcium Mobilization Assay Results of Bivalent Ligand 1a and CXCR4 Monovalent Control 3a^a.

	antibody binding	Ca²⁺ flux
compd	IC₅₀ (nM)	IC₅₀ (nM)
IT1t^b	7.40 ± 3.80	32.5 ± 8.8
1a	3600 ± 300	8300 ± 2300
3a	69000 ± 24000	53000 ± 6600

The values are the mean ± SEM of three independent experiments. The CXCR4 antibody-binding assay was performed on CHO-CXCR4 cells. The antagonist calcium assay was conducted on HOS-CXCR4 cells.

Data adopted from ref (15).

Table 2. CXCR4 Antibody Binding Assay and Calcium Mobilization Assay Results of Bivalent Ligand 1a and CXCR4 Monovalent Control 3aa.

Table 2. CXCR4 Antibody Binding Assay and Calcium Mobilization Assay Results of Bivalent Ligand 1a and CXCR4 Monovalent Control 3a^a.