Concentration-inhibition curves of 14a (A) and 14b (B) at human recombinant
soluble
CD73 (red circles), human membrane-bound CD73 natively expressed in
the triple negative breast cancer cell line MDA-MB-231 (blue circles),
and rat recombinant soluble CD73 (green circles). Data points are
means ± standard error (SEM) from 3 separate experiments. Determined Ki values for 14a: MDA-MB-231 cells, 4.59 nM; human soluble CD73, 2.98 nM; rat soluble
CD73, 26.0 nM. Determined Ki value for 14b: human soluble CD73, 12.6 nM.