Figure EV4. (related to Fig 6): STING Tyr phosphorylation, signaling protein interaction, and phosphorylation.

1. Silver staining of EGFR and recombinant STING used in the experiments of Fig 6A.
2. Silver staining of EGFR and recombinant STING mutants shown in Fig 6B.
3. The degree of modification was determined by plotting chromatograms for both the unmodified and modified forms of the Y245 peptides. Chromatograms for the VYSNSIpYELLENGQR peptides are shown.
4. STING Y244F‐Myc and STING WT‐Myc recombinant stable cell lines in primary STING^−/− MEF were treated with cGAMP for 2 h; then, the cell lysates were subjected to Myc pull‐down by Myc trap beads. The pull‐down products were analyzed by Western blot with the indicated antibodies.
5. The same cell lines as in (D) were treated with cGAMP for the indicated time; the cell lysates were used for Western blot with p‐S365 STING antibody.
6. WT and EGFR^−/− HeLa cells were treated with Torin (10 μM) and CQ (Chloroquine, 20 μM) for 1 h and then stimulated with cGAMP for 1 h, where indicated. Cell lysates were subjected to Western blot analysis via LC3II antibody.