Skip to main content
. 2020 Sep 20;39(22):e106249. doi: 10.15252/embj.2020106249

Figure EV1. Variation in hub architecture in some T. agilis sub‐volumes.

Figure EV1

  1. Transverse 2D slices through central cartwheel STA of T. agilis 45% class, which comprises 25 and 20% sub‐classes (see E), at indicated height from proximal (0 nm) to distal (18.0 nm). The pink circles mark spokes with CID (12.0 nm, dashed line) or without CID (2.0 nm, solid line), as represented in (B); dashed box in the 4.0 nm slice indicates longitudinal section shown in (C). Schematic on top illustrates the area used to generate the 3D maps of the central cartwheel.
  2. Transverse views of central cartwheel STA 3D map of T. agilis 45% class. The hub diameter is 22.7 nm ± 0.2 nm (N = 3) with 9 emanating spoke densities, either at a level where the CID is present (dashed circle) or absent (solid circle), as indicated in (A).
  3. 2D longitudinal view of central cartwheel STA of T. agilis 45% class delineated by a dashed box in (A). Arrowheads denote position of line scans along the vertical axis at the level of the CID (red) and the hub (pink), with corresponding normalized pixel intensities in arbitrary units (AU). The plot profiles are shifted horizontally relative to each other for better visibility. The distance between hub densities alternates between 3.2 nm (N = 1) and 6.0 ± 0.3 nm (N = 2); maxima are indicated by dashed pink lines. Dashed white line indicates offset of two superimposed hub units. The average distance between two CID elements is 15.2 nm (N = 1; dashed red line). The middle hub density that comprises only one unit and lacks a neighboring CID is indicated by a dashed arrow.
  4. Longitudinal view of central cartwheel STA of T. agilis 45% class at lower contour level than in (B). Note densities bridging successive hubs vertically (arrows), as well as proximal location of CID relative to the spoke density axis, resulting in a vertical offset of 1.2 ± 0 nm (N = 2; arrowhead). Note also absence of CID in middle hub element comprising a single unit.
  5. The spacing between the spoke density emanating from a double hub unit and the spoke density emanating from a single hub unit distal to it is 8.0 nm in the 25% class and 7.1 nm in the 20% class (both N = 1). By contrast, the spacing between the spoke density emanating from a double hub unit and the spoke density emanating from a single hub unit proximal to it is 8.7 nm in the 25% class and 7.6 nm in the 20% class (both N = 1).
  6. Distribution of sub‐volumes of the 55% (black circle) and 45% (white circle) classes along five T. agilis centrioles; areas with neither black nor white circle could not be clearly assigned to either class. Note individual centrioles constituted of mostly one of the two classes, and others where the two classes are mixed without an apparent pattern, indicating that the distribution of 55% and 45% classes is not stereotyped along the centriole.