Figure EV4. CrSAS‐6[6HR] fitting in different cartwheel STA maps.

• A
  Ribbon representation of CrSAS‐6[6HR] homodimers (top) shown magnified in side view (left) and end‐on view (right) that were used for rigid‐body fitting into the 3D map from T. mirabilis 36% class (bottom), with dashed line indicating offset between two superimposed homodimers. Individual layers of homodimers are indicated in distinct colors for clarity. In this and all other panels of this figure, dashed box indicates longitudinal section through hub element (left), and double chevron indicates viewing direction of longitudinal external views (right).
• B–D
  CrSAS‐6[6HR] single or double rings in register (ribbon diagram represented in different shades for clarity) fitted into the 3D maps from the T. mirabilis 64% class (B), T. spp. (C) and the T. agilis 55% class (D). Single ring fitting is shown on top, double ring fitting at the bottom. Note unaccounted densities in the hub upon fitting of single rings in all cryo‐ET maps (arrowheads, B‐D), whereas all maps readily accommodate double CrSAS‐6[6HR] rings at the hub element, although the coiled‐coil elements extend slightly beyond the spoke densities. Note also that one coiled‐coil in the T. mirabilis 64% class sticks out of the density entirely (B, bottom).