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. 2020 Sep 20;39(22):e106249. doi: 10.15252/embj.2020106249

Figure EV5. Superimposed rings of SAS‐6 may be offset with respect to one another.

Figure EV5

  • A, B
    (Left) Ribbon representation of CrSAS‐6[6HR] homodimers computationally assembled into double rings (rings represented in different colors for clarity) with spokes in register (A) or with a 6.5° offset turning the proximal ring clockwise and moved 0.4 nm closer to one another (B); transverse (top) and longitudinal view (bottom). (Right top) Magnified views of two vertically superimposed homodimers assembled in a ring (ribbon diagram), with dashed lines indicating the offset angle between the two coiled‐coil axes. (Right bottom) Surface representation of interface; note the increased complementarity in (B). Dashed lines indicate offset between rings. Distances were measured on the ribbon diagram.
  • C, D
    CrSAS‐6[6HR] offset double rings (shown in B and represented in different colors for clarity) fitted into 3D map from T. mirabilis 36% class (C) and T. agilis 45% class (D), with dashed line indicating offset between rings. Dashed box indicates longitudinal section through hub element (left), double chevron viewing point of longitudinal external views (right).
  • E
    Schematic illustrating processing reported in (F‐H): After linearization of the cartwheel STA, a longitudinal section in the Z‐direction (blue line) is shown at the level of the spokes (represented in pink).
  • F–H
    Longitudinal section as explained in (E) for T. mirabilis 36 and 64% classes (F), T. agilis 45 and 55% classes (G) and T. spp. (H) at the level of spoke emergence from the hub. Note offset visible by the elliptic shape of individual spokes and highlighted by a dashed line.
  • A
    Longitudinal section as explained in (E) for in vitro assembled CrSAS‐6[NL] stacks; spokes from consecutive stacks are almost in register. Note that in (F‐H) the spokes of double hub elements cannot be fully resolved, whereas they appear as individual units in (I).