Skip to main content
. 2020 Oct 1;39(22):e104941. doi: 10.15252/embj.2020104941

Figure 4. PAP8 is functional in both the chloroplast and the nucleus.

Figure 4

  • A, B
    (A) Transient expression of PAP8NLSm5‐GFP mutated in the NLS as described in Fig EV4A. The circle marks the position of the nucleus (N) as observed with DIC in (B); yellow arrowheads show stromules (str). Scale bars equal 20 μm.
  • C–G
    Pictures of representative genotypes obtained in the functional complementation test of pap8‐1 using pP8::PAP8‐NLSm5 (C, F) or pP8::PAP8ΔcTP (G) both constructions without GFP tags; ø indicates pap8‐1 without any PAP8 transgene. (D–G) Pictures using Keyence technology of plants with genotypes as labelled; transgenes expressed using the 1.1‐kb PAP8 promoter. Scale bars equal 1 mm.