Figure EV4. (Related to Fig 4) Hemi‐complementation test in pap8‐1 .

• A
  Genetic alterations of the pap8 ^NLSm5 allele. Altered codons and corresponding changes in amino acids (central line) are in magenta; the sign “>” indicates aa replacement at the same position.
• B
  Bar graph representing the albino segregation ratios in doubly heterozygous transgenic lines (pap8-1/+; TG/−) obtained with pPAP8::PAP8NLSm5 (NLSm5: pAi10) and pPAP8::PAP8∆cTP (∆cTP: pAi15). pap8‐1/+ used as control; n, total number of recorded plants. Segregation pattern were tested using ε‐test with null hypothesis set to p0 = 1/16 (greening complementation) for NLSm5 and p0 = 1/4 (no complementation) for ∆cTP (see data source); black *, outliers correspond to the samples that did not pass the statistical test; red * indicate chosen genotypes for follow‐up studies.
• C
  Phenotype of pap8‐1 transformed with pPAP8::PAP8^NLSm5. WT, 5‐week-old Col‐0 control; [DG], delayed greening phenotype observed for the partial rescue of pap8‐1 mutant expressing PAP8^NLSm5 under its endogenous promoter; pictures depict three 15‐week-old plants in which the alteration of the greening corresponds to the emergence of white leaves that slowly acquire the photosynthetic apparatus; plants #7, #31 and #57 are siblings of the same genotype (Ai10#34). Scale bars equal 10 mm.

Source data are available online for this figure.