Figure 7. Temporal resolution of protein content in seedlings during the dark‐to‐light transition.

• A
  Immuno‐blots showing the levels of PAP8, PAP5, histone H3 (H3), Rubisco (RbcL) and HY5; D, dark; Ph, photomorphogenic growth conditions from germination on. *, HY5 is detected as a modified form (+6 kDa); the arrows indicate the two different post‐translational modified forms of HY5 for which accumulation occurs upon light exposure.
• B
  Relative protein contents normalized to histone H3 during the transition from skotomorphogenesis to photomorphogenesis, we propose a phase of photo‐initiation corresponding to the 5 min of light allowing PAP8 and PAP5 to rise to nearly 100% of their maximum. The first macroscopic signs of greening are indicated around 3 h, while the photosynthetic apparatus bursts at 8 h to be fully accumulated at 48 h.

Source data are available online for this figure.