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. 2020 Nov 10;13:883–895. doi: 10.2147/JIR.S278556

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© 2020 Huang et al.

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Effect of SA inhibit IL-1β induced extracellular matrix degradation in nucleus pulposus cells. (A) Protein expressions of collagen II, aggrecan, MMP13, and ADAMTS5 in NP cells treated as above were evaluated by Western blot. (B–E) Quantification of immunoblots of collagen II, aggrecan, MMP13, and ADAMTS5. (F) The representative collagen II was detected by the immunofluorescence combined with DAPI staining for nuclei (original magnification ×400, scale bar: 25 μm). (G) The representative MMP13 were detected by the immunofluorescence combined with DAPI staining for nuclei (original magnification ×400, scale bar: 25 μm). (H) The fluorescence intensity of collagen II was analyzed by Image J. (I) The fluorescence intensity of MMP13 was analyzed by Image J. All experiments were performed at least three times, and the data in the figures represent the mean ± S.D. ^##P < 0.01 compared with control group. **P < 0.01 compared with IL‐1β group.