Figure 4.

Reduced Tpm-based inhibition persists in acetylated actin-containing RTFs. A, representative images illustrating immeasurable (pCa 9) and equivalent (pCa 4) movement of control and acetylated actin-containing RTFs by overlaying the first frame of a 17–20-s movie (white) with a summative image of total motion (yellow). Scale bar, 7 μm. B, percentage of filaments moving (left; n = 9) and average velocities (right; n = 2) of control (black; 40.1 ± 3.9% and 1.19 ± 0.21 μm/s, respectively) and acetylated (gray; 40.5 ± 3.4% and 1.11 ± 0.17 μm/s, respectively) RTFs at pCa 4 were indistinguishable (two-tailed t test). C, percentage of filaments moving (left; n = 7) and average velocities (right; n = 2) of acetylated (24.75 ± 1.63% and 0.27 ± 0.01 μm/s, respectively) RTFs were significantly greater than control (20.11 ± 1.45% and 0.18 ± 0.02 μm/s, respectively) at pCa 6.5 (two-tailed t test; *, p < 0.05).