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. 2020 Sep 14;295(46):15454–15463. doi: 10.1074/jbc.RA120.014814

Figure 2.

Figure 2.

The E. coli Fur has a bright red color when expressed in the E. coli iscA/sufA mutant cells. Recombinant E. coli Fur was expressed in the E. coli WT and the E. coli iscA/sufA mutant cells grown in LB medium under aerobic conditions. A, UV-visible absorption spectra of purified Fur proteins. Spectrum 1, Fur protein purified from WT E. coli cells. Spectrum 2, Fur protein purified from the iscA/sufA mutant cells. The protein (100 μm) was dissolved in buffer containing 20 mm Tris (pH 8.0) and 500 mm NaCl. Inset, photographs of purified Fur proteins from WT (panel 1) and the iscA/sufA mutant (panel 2) cells. B, UV-visible absorption spectrum of the Fur protein purified from WT E. coli cells. Same as in A, except the y axis is expanded by 8-fold. C, SDS-PAGE gel of purified Fur proteins from WT E. coli cells (lane 1) and from the iscA/sufA mutant cells (lane 2). Lane M, molecular mass ladder. D, EPR spectra of the E. coli Fur protein purified from the iscA/sufA mutant cells. The Fur protein (500 μm) was reduced with freshly prepared sodium dithionite (10 mm) and immediately frozen in liquid nitrogen until the EPR measurement.