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. 2020 Sep 9;295(46):15727–15741. doi: 10.1074/jbc.RA120.014690

Figure 4.

Figure 4.

SCARA5 recognizes both L-ferritin and H-ferritin. A, FACS data showed that both FITC-labeled L-ferritin and H-ferritin bound to the hSCARA5 transfected cells, but not the hSCARA5 (E486A)-transfected cells. B, fluorescent images showed that both L-ferritin and H-ferritin could be internalized by the hSCARA5-transfected cells, but not the hSCARA5 (E486A)-transfected cells (bar, 25 μm). C, ELISA data showed that both L-ferritin and H-ferritin could bind to the SRCR domain of hSCARA5, but not to the SRCR (E486A) mutant. D, FLAG tag pulldown assays showed that both L-ferritin and H-ferritin could interact with the SRCR domain of hSCARA5. E, FACS data showed that the unlabeled L-ferritin could block the binding of the FITC-labeled H-ferritin by the SCARA5-transfected cells almost completely. F, FACS data showed that unlabeled H-ferritin could partially block the binding of the FITC-labeled L-ferritin by the SCARA5-transfected cells. Error bars, S.D.