Figure 6.

Interactions of L-ferritin with the SR-A members. A, FACS data (left) and fluorescent images (right) showed that human SCARA1, MARCO, and SCARA5 could bind to L-ferritin, whereas SCARA3, SCARA4, SCARA1ΔSRCR, SCARA1 (D376A/D377A), and CD163 had no binding to L-ferritin (bar, 25 μm). B, ELISA data showed that the SRCR domain of human SCARA5 had higher binding affinity with L-ferritin than the SRCR domains of human SCARA1 and MARCO. C, schematic model for the recognition of ferritin by SCARA5 on the cell surface. Error bars, S.D.