Table 1.
X-ray data collection and processing
| Protein | SRCR (hSCARA5) | SRCR (mSCARA5) |
|---|---|---|
| Diffraction source | BL18U1, SSRF | BL18U1, SSRF |
| Wavelength (Å) | 0.98 | 0.98 |
| Detector | Dectris PILATUS 6M | Dectris PILATUS 6M |
| Temperature (K) | 100 | 100 |
| Space group | P 212121 | P31 |
| a, b, c (Å) | 38.2, 48.8, 70.8 | 39.8, 39.8, 132.2 |
| α, β, γ (degrees) | 90, 90, 90 | 90, 90, 120 |
| Resolution range (Å) | 28.65–1.65 (1.71–1.65)a | 27.14–2.30 (2.38–2.30) |
| Rmerge | 0.082 (0.393) | 0.095 (0.326) |
| Rpim | 0.026 (0.125) | 0.043 (0.117) |
| No. of unique reflections | 15,075 (1465) | 10,380 (1040) |
| Completeness (%) | 99.59 (98.80) | 99.43 (99.11) |
| Multiplicity | 12.7 (12.7) | 10.3 (9.9) |
| Mean I/σ(I) | 29.03 (6.88) | 26.22 (7.00) |
| Overall B factor from Wilson plot (Å2) | 13.69 | 38.26 |
aValues in parentheses are for the highest-resolution shell.