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ADAMTSL2 secretion required O-fucosylation but not N-glycan fucosylation. Plasmids encoding ADAMTSL2-MycHis6 and hIgG were co-transfected into (A) Lec13-CHO cells grown in the presence (+) or absence (−) of 1 mm l-fucose or (B) into Lec1-CHO cells and Pro5-CHO cells. The medium and cell lysates were analyzed by Western blots probed with anti-myc (red) and anti-IgG (green) antibodies.
